Part:BBa_J119313:Experience

pClone Blue contains a promoter in reverse orientation between BsaI sites that use CGAC and GCGG sticky ends. The promoter drives transcription of a GFP gene in reverse orientation preceded by a RBS. Insertion of a functional promoter will lead to transcription of the AmilCP Blue gene preceded by an RBS. A pClone Blue negative control assembly produces colonies that display GFP fluorescence under UV light (left). GGA with a functional promoter (center) yields a mixture of green fluorescent colonies containing the starting pClone Green plasmid and colonies that are blue under with either overhead white light (center left) or on a white light box (center right). GGA with a non-functional or inactive promoter yields a mixture of green fluorescent colonies containing the starting pClone Green plasmid and non-fluorescent non-blue colonies containing the non-functional or inactive promoter (right).

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